rabbit anti pakt (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc rabbit anti pakt
Rabbit Anti Pakt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 32597 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pakt+(ser473/Phospho-Akt+(Ser473)+Antibody/pm41934916-54-5-7
Average 99 stars, based on 32597 article reviews

rabbit anti pakt - by Bioz Stars, 2026-09

99/100 stars

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Incubation:

Article Title: VA1213, a selective COX-2 inhibitor, exhibits antitumor activity by suppressing EGFR, AKT, and ERK1/2 phosphorylation.
Article Snippet: Proteins were transferred onto a nitrocellulose membrane (iBlot 2 Transfer Stacks, nitrocellulose, regular size from Thermo Fisher Scientific, Inc.). .. Next, membranes were blocked with 5 % non-fat dried milk for 1 h at room temperature, and then incubated at 4 °C overnight with primary antibody including anti-COX-2 (mouse, 1:1000) provided by Cayman Chemical, Ann Arbor, MI USA), CDK2 (rabbit, 1:1000), CDK4 (rabbit, 1:1000), Cyclin D1 (rabbit, 1:1000), Cyclin D3 (rabbit, 1:1000), p21 (rabbit, 1:1000), caspase 3 (rabbit, 1:1000), cleaved caspase-3 (Asp175) (rabbit, 1:1000), pERK1/2 (rabbit 1:2000), ERK1/2 (rabbit, 1:1000), pAKT (ser473) (rabbit 1:1000), anti-AKT (mouse 1:2000), pEGFR (rabbit, 1:1000) and EGFR (rabbit, 1:1000) purchased from Cell Signaling Technology (Danvers, MA, USA). .. Anti-βactin antibody (mouse, 1:10,000) was from Merck KGaA (Darmstadt, Germany).

Cell Characterization:

Article Title: Exercise preconditioning prevents immobilization-induced skeletal muscle atrophy by activating Prmt1-p38/ATF2-Sesn1 signaling axis in C57BL/6J mice
Article Snippet: .. The antibodies are listed below: aDMA (Anti-Asymmetric Di-Methyl Arginine Motif) (1:1 500, Rabbit, Cell Signal Tech, USA), Akt (protein kinase B) (1:2 000, Mouse, Proteintech, USA), pAkt-Ser473 (1:2 000, Rabbit, Cell Signal Tech, USA), AMPKα2 (1:2 000, Rabbit, Cell Signal Tech, USA), pAMPK-Thr172 (1:2 000, Rabbit, Cell Signal Tech, USA), ATF2 (1:2 000, Rabbit, Proteintech, USA), Atrogin-1 (FBXO32) (1:15 000, Mouse, Proteintech, USA), FoxO3a (Forkhead box O3) (1:1 000, Mouse, Proteintech, USA), pFoxO3a-Ser315 (1:2 000, Rabbit, Proteintech, USA), GAPDH (1:5 000, Rabbit, Utibody, CN), IGF-1 (insulin-like growth factor 1) (1:2 000, Mouse, Proteintech, USA), LaminB (1:2 000, Rabbit, Abcam, USA), MEF2 (myocyte enhancer factor 2) (1:2 000, Rabbit, Proteintech, USA), Myf5 (myogenic factor 5) (1:2 000, Rabbit, Abclonal, CN), MyoD (myogenic differentiation antigen) (1:2 000, Rabbit, Proteintech, USA), MyoG (myogenin) (1:2 000, Rabbit, Proteintech, USA). mTOR (mammalian target of rapamycin) (1:2 000, Rabbit, Cell Signal Tech, USA), p38 (1:2 000, Rabbit, WANLEIBIO, CN), p-p38-Thr180/Tyr182 (1:2 000, Rabbit, WANLEIBIO, CN), PGC-1α (1:1 000, Rabbit, Abcam, USA), Prmt1 (1:2 000, Rabbit, Cell Signal Tech, USA), Raptor (1:1 000, Rabbit, Cell Signal Tech, USA), Sesn1 (1:1 000, Rabbit, HUABIO, CN), Sesn1 (1:1 000, Rabbit, Abcam, USA), TRIM63 (MuRF1) (1:2 000, Rabbit, Proteintech, USA). ..

Pyrolysis Gas Chromatography:

Transduction:

Article Title: PKC-eta promotes breast cancer metastasis by regulating the Hippo-YAP signaling pathway.
Article Snippet: .. The antibodies used in this study were against YAP (CST, #14074), MST1 (CST, #3682), pMST1 (CST, #3681), LATS1 (CST, #3477), 14-3-3 ζ/δ (CST, #7413), pLATS1 (CST, #8654), pYAP (Ser127) (CST, Signal Transduction and Targeted Therapy (2026) 11:58 #13008), pYAP (Ser109) (CST, #53749), pYAP (Ser397) (CST, #13619), pTAZ (Ser89) (CST, #75275), TAZ (CST, #83669), PTEN (CST, #9559) Lamin B1 (CST, #13435), AKT (pan) (CST, #4691), AXL (CST, #8661), Pan-TEAD (CST, #13295), CYR61 (CST, 14479), IGFBP3 (CST, #25864), HA-Tag (CST, #3724), FLAG (CST, #14793), pAKT (Ser473) (CST, #4060), and Anti-rabbit IgG (CST, # 7074). .. Epithelial–Mesenchymal Transition (EMT) Antibody Sampler Kit, CST, #9782 [(Vimentin (D21H3), Snail (C15D3), Slug (C19G7), ZEB1 (D80D3), N-cadherin (D4R1H), ZO-1 (D7D12), E-cadherin (24E10)]. pYAP (Ser128) (Thermo Fisher Scientific, # PA5-117264), PKCη (Santa Cruz, #sc215), PKCε (BD Biosciences, #610085), PKCδ (BD Biosciences, #610398), PKCα (BD Biosciences, #610108), PKCζ (Abcam #ab59364), PKCη (Abcam #ab179524), EpCAM (Abcam, ab223582), and β-actin (ICN Biomedicals Inc., 691001) were used.

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